Division: Tokyo University of Agriculture
Main Research: Initiative 3 "The Forestry Model of Sustainable Humanosphere"
Project Title: Tree breeding for sustainable industrial forests
Research Theme:
- Studies on plant cell walls
- Tree breeding for reforestation
- Field trials of transgenic trees related to the synthesis and degradation of cellulose
References:
- T. Hayashi: The Science and Lore of the Plant Cell Wall - Biosynthesis, Structure and Function, BrownWalker Press, Florida USA (2006).
- T. Hayashi, K. Yoshida, Y.W. Park, T. Konishi and K. Baba: Cellulose metabolism in plants, International Review of Cytology, 247, 1-34 (2005).
2007 G-COE Research Outcome:
- A Alonso-Simon, P Garcia-Angulo, AE Encina, JM Alvarez, JL Acebes and T Hayashi 2007 Increase in XET activity in bean ( Phaseolus vulgaris L.) cells habituated to dichlobenil, Planta, 226: 765-771．
- N Nishikubo, T Awano, A Banasiak, V Bourquin, F Ibatullin, R Funada, H Brumer, TT Teeri, T Hayashi, B Sundberg and EJ Mellerowicz 2007 Xyloglucan Endo-transglycosylase (XET) Functions in Gelatinous Layers of Tension Wood Fibers in Poplar—A Glimpse into the Mechanism of the Balancing Act of Trees, Plant Cell Physiol, 48: 843-855.
- BR Urbanowicz, AB Bennett, E del Campillo, C Catalá, T Hayashi, B Henrissat, H Höfte, SJ McQueen-Mason, SE Patterson, O Shoseyov, TT Teeri, and JKC Rose 2007 Structural organization and a standardized nomenclature for plant endo-1,4--glucanases (cellulases) of glycosyl hydrolase family 9. Plant Physiology, 144: 1693-1696．
- T Hayashi, YW Park, A Isogai and T Nomura 2008 Cross-linking of plant cell walls with dehydrated fructose by smoke-heat treatment. Journal of Wood Science, 54: 90-93.
List of Academic Presentations during 2007:
- T. Hayashi, M. Takeuchi, Y.W. Park, T. Kaku, M. Yoshida, T. Awano, R. Kaida, K. Baba: Xyloglucan creates tensile stress in the secondary wall. XI Cell Wall Meeting 15th August 2007.
- T. Hayashi Tropical trees in Southeast Asia - A reformation scenario from deforestation, Plant Science and Genetics seminar, the Hebrew University of Jerusalem, 18th December 2007.
2008 G-COE Research Outcome:
- T Hayashi, YW Park, A Isogai and T Nomura, Cross-linking of plant cell walls with dehydrated fructose by smoke-heat treatment. J Wood Sci, 54: 90-93 (2008)
- T Taniguchi, Y Ohmiya, M, Kurita, M Tsubomura, T Kondo, YW Park, K Baba, T Hayashi, Biosafety assessment of transgenic poplars overexpressing xyloglucanase (AaXEG2) prior to field trials, J Wood Sci, 54:408-413 (2008)
- R Kaida, T Hayashi, TS Kaneko, Purple acid phosphatase in the walls of tobacco cells, Phytochem, 69: 2546-2551 (2008)
- T Takabe T, A Uchida A, F Shinagawa, Y Terada, H Kajita, Y Tanaka, T Takabe, T Hayashi, T Kawai, T Takabe, Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica enhances growth rate as well as abiotic stress tolerance of poplar plants, Plant Growth Reg, 56: 265-273 (2008)
- T Takabe T, A Uchida A, F Shinagawa, Y Terada, H Kajita, Y Tanaka, T Takabe, T Hayashi, T Kawai, T Takabe, Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica enhances growth rate as well as abiotic stress tolerance of poplar plants, Plant Growth Reg, 56: 265-273 (2008)
- S Hartati, E Sudarmonowati E, YW Park YW, T Kaku, R Kaida, K Baba, T Hayashi, Overexpression of poplar cellulase accelerates growth and disturbs the closing movements of leaves in sengon, Plant Physiol, 147: 552-561 (2008)
- H Ikegaya, T Hayashi, T Kaku, K Twata, S Sonobe, T Shimmen: Presence of xyloglucan-like polysaccharide in Spirogyra and possible involvement in cell-cell attachment, Phycological Res, 56: 216-222 (2008)
- T Hayashi, R Kaida, T Kaku, K Baba, Enhancement of saccharification by overexpression of various endoglycanase in poplar, GEO Giacaglia ed, pp 145-149, UNINDU, Taubate, Brasil (2008)
- K Ozaki, A Uchida, T Takabe, F Shinagawa, Y Tanaka, T Takabe, T Hayashi, T Hattori, AK. Raid, T Takabe, Enrichment of sugar content in melon fruits by hydrogen peroxide treatment, J Plant Physiol, 166: 569-578 (2009)
List of Academic Presentations during 2008:
- T Hayashi: Enhancement of saccharification by overexpression of various endoglycanase in poplar, International Symposium on Clean Energy for the World, Ethanol Biodiesel and Nayural Gas, Ubatuba, Brasil, December 7-10, 2008 (Invited)
- T Hayashi: Transgenic poplars, Plant feedstock seminar, Joint BioEnergy Institute, Emeryville, USA, 15th December (2008)
- T Hayashi: Transgenic poplars, Plant biology seminar, Sao Paulo University, Sao Paulo, Brasil, 12th December (2008)
- T Hayashi: Proposed strategies and prospects for Riau biosphere reserve, Workshop on Riau biosphere researve, Pekanbaru, Indonesia, February 20, 2009
- T Hayashi: Loosening xyloglucan accelerates the enzymatic hydrolysis of cellulose in wood, Nissan International Tree Biology, Tokyo, February 26, 2009 (Invited)
2009 G-COE Research Outcome:
- Hayashi T., Kaida R., Kaku T., Baba K. (2010) Loosening xyloglucan prevents tensile stress in tree stem bending but accelerates the enzymatic degradation of cellulose. Russian J. Plant Physiol. 57, 316-320.
- Kaida R., Kaku T., Baba K., Oyadomari M., Watanabe T., Nishida K., Kanaya T., Shani Z., Shoseyov O., Hayashi T. (2009) Loosening xyloglucan accelerates the enzymatic degradation of cellulose in wood. Mol. Plant 2, 904-909.
- Kaida R., Kaku T., Baba K., Hayashi T. (2009) Enzymatic saccharification and ethanol production of Acacia mangium and Paraserianthes falcataria wood, and oil palm trunk. J. Wood Sci. 55, 381-386.
- Tomomi Kaku, Satoshi Serada, Kei’ichi Baba, Fumio Tanaka, Takahisa Hayashi (2009) Proteomic analysis of the G-layer in poplar tension wood, J Wood Sci 55:250–257.
- Kaida R., Kaku T., Baba K., Hartati S., Sudarmonowati E., Hayashi T. (2009) Enhancement of saccharification by overexpression of poplar cellulase in sengon. J. Wood Sci. 55,435-440.
- Kaida R., Satoh Y., Bulone V., Yamada Y., Kaku T., Hayashi T., Kaneko T. (2009) Activation of -glucan synthases by wall-bound purple acid phosphatase in tobacco cells. Plant Physiol. 150, 1822-1830.
- Baba K., Park W.P., Kaku T., Kaida R., Takeuchi M., Yoshida M., Hosoo Y., Ojio Y., Okuyama T., Taniguchi T., Ohmiya Y., Kondo T., Shani J., Shoseyov O., Awano T., Serada S, Norioka N., Norioka S., Hayashi T. (2009) Xyloglucan for generating tensile stress to bend tree stem. Mol. Plant 2, 893-903.
- Hayashi T., Kaida R., Mistuda N., Ohme-Takagi M., Nishikubo N., Kidou S., Yoshida K. (2009) Enhancing primary raw materials for biofuels. In Biomass to Biofuels, Wiley, pp 459-489.
List of Academic Presentations during 2009:
- T Hayashi: Bioethanol Revolution, 2rd Humanosphere Science school, Cibinong, Indonesia, March 26-27 (2009)
- T Hayashi: Case study on bioethanol production in Riau, 3rd Humanosphere Science school, Pekanbaru, Indonesia, August 4-5 (2009)
- T Hayashi: Transgenic trees overexpressing AaXEG, Plant Science and Genetic Seminar, the Hebrew University of Jerusalem, November 25 (2009)
- T Hayashi: Our strategy on Riau Biosphere Reserve, International Workshop on Sustainable Forest Tree Plantation in the Giam Siak-Bukit Batu Biosphere Reserve of Indonesia, Kyoto, Japan, February 4 (2010)